The ultimate success or failure of a library preparation strongly depends on using an accurately quantified amount of input DNA. We ask for 2 ug of genomic DNA for standard PCR-free genomic libraries. Correct quantification of genomic DNA is essential and should ideally be done by fluorimetry. Please feel free to contact Muriel Fragnière, if you want to use our Qubit 2.0 system.
The ultimate success or failure of a library preparation strongly depends on using intact RNA and an accurately quantified amount of input RNA. We ask for 2 ug of total RNA for standard illumina stranded mRNA libraries. You need to document the integrity of the RNA by providing us with some documentation of your QC (e.g. Bioanalyzer RIN number). Please feel free to contact Muriel Fragnière, if you want to perform QC of your RNA in our lab.
The PacBio Sequel is a single molecule sequencing instrument that enables very long read lengths. DNA libraries for the PacBio require an input of >10 ug of high molecular weight DNA. Genomic DNA should be >50 kb in size and special attention must be given to avoid any unnessary strand breaks (e.g. during freeze-thaw cycles). If you are interested in a PacBio experiment, please discuss the project with us well in advance (prior to isolation of the nucleic acids). This will ensure an optimal quality of your PacBio sequencing data.